Chromatography Technique
Chromatography Technique
it is based on Greek word, "kroma" means colour. This separation was first used for the separation of colour, hence it got this name.
Chromatography is a technique used to separate those solutes of a mixture which are soluble in the same solvent. It is used to separate the colored components of a natural dye. The separation of different components of a mixture depends on their solubilities in the same solvent.
It is a modern technique used for the separation of a mixture of substances which when separated, formed distinct coloured rings. These zones are named as chromatograms. These days, chromatography is applied to coloured as well as colourless substances for analysis.
Principle :-
The principle on which paper chromatography work is very simple. The pulp in the filter paper used for chromatography, is composed of thin threads acting as cappillaries. Different soluble dyes move upwards at different speeds and to different levels, by capillary action. In the laboratory, a fine quality of ordinary filter paper can also be used.
Working :-
Example: Dye present in ink can be separated from ink by paper chromatography. The ink that is used has water as the solvent and dye is mixed in it to provide colour.
Depending upon the nature of the stationary and the mobile phases, the different types of chromatographic techniques commonly used are :-
Application of Chromatography:
Assertion : Moving phase is liquid and stationary phase is solid in paper chromatography. Reason : Paper chromatography is used for analysis of polar organic compounds. | |||
| Right Option : B | |||
| View Explanation | |||
Chromatography is used to separate the ________________ components of a natural dye. | |||
| Right Option : A | |||
| View Explanation | |||
On the basis of difference in which of the following physical properties are the components of mixture gets separated in chromatography? | |||
| Right Option : C | |||
| View Explanation | |||
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